Fast On-Line Trypsin Digestion

 

1-4 minutes trypsin digestion using a high-efficiency trypsin column

 

The benefits of using Perfinity iDP with the immobilized trypsin column are:



  1. Fast trypsin digestion in 1 to 4 minutes

  2. Continuous sample analysis on reusable trypsin column

  3. Reduced chymotrypsin activity and deamidation Insulin was digested within 1 minute using the trypsin column as shown in the figure at right.

     

Insulin was digested within 1 minute using the trypsin column as shown in the figure at right.

* Optimal digestion time depends on proteins.

 

Optimized Perfinity iDP digestion buffer

 

The optimized trypsin digestion buffer in combination with the trypsin column enables highly efficient digestion.

The figure to the right shows a comparison between the buffer. The optimized digestion buffer provided a higher peptide recovery rate with a short digestion time.
 
 
 
 

Increased throughput by parallel sample processing with two desalting columns

 

Two desalting columns allow parallel processing of samples. One desalting column is used to trap digested peptides, while peptides from the previously injected sample are eluted from the other desalting column.

This parallel sample processing allows analysis of up to 200 samples/day.



The figure to the right is an example of parallel sample processing, which increases throughput while maintaining reproducibility.
 

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