Fast Simultaneous Analysis & Superb Detection Selectivity

Fast Simultaneous Analysis of 38 Amino Acid Components in Nine Minutes

 

Rapid separation combined with LC/MS detection results in the simultaneous analysis of 38 biogenic amino acid components in just nine minutes. The ability to analyze up to 96 samples in 24 hours ensures exceptional performance for multi-sample analysis.
The increased speed supports new applications, such as real-time amino acid composition analysis in culture fluid, that were difficult to handle using conventional techniques.

 

About 18 Times Faster
 

Biogenic amino acid analysis using a combination of ion-exchange chromatography and postcolumn derivatization requires over two hours to achieve multicomponent separation. In contrast, UF-Amino Station can analyze 38 biogenic amino acid components in just nine minutes. This analysis time is about 18 times shorter, enabling a significant improvement in throughput and productivity.

 

LC/MS Offers Superb Detection Selectivity

 

A method using precolumn derivatization and UV detection combined with reversed-phase column separation can be used to reduce analysis times. However, it may not achieve adequate separation and the reliability of the analysis results may be limited in cases with numerous target components, such as biogenic amino acids, or if the sample contains many impurities. By using an LC/MS instrument as the detector, UF-Amino Station permits highly reliable analysis even when analyzing a large number of target components or if the sample contains a complex matrix.

 

LC/MS Permits Qualification and Quantitation of Peaks that Cannot be Separated by HPLC
 

Since analysis by pre-column derivatization and UV detection lacks selectivity, quantitation can be adversely affected by inadequate separation on the column. As shown in the diagram below, commercially available skim milk powder can be difficult to analyze via pre-column derivatization using PTC and UV detection because of the difficulty in the separation of multiple amino acid components and impurities. UF-Amino Station utilizes LC/MS technology for quantitation by using particular mass to charge ratios for each amino acid.

By utilizing LC/MS the UF-Amino Station delivers the benefits of a highly selective detector, allowing for better analytical results.

 

 

 

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