Western blotting (3)
Micro-scale reagent delivery
Delivers picoliter volumes of reagent to a target surface using piezoelectric technology.
Application: Western blotting
Comparison between conventional Western blots and the new CHIP approach.
- Results in equivalent data between the 2 approaches.
- The CHIP is far quicker and uses less reagent than the conventional method (more cost effective).
- The true value is in the ability to view multiple anti-bodies in one blot - it is easier and quicker to interpret and visualize.
- Conventional Western blotting.
Three individual blots used for each anti-body.
- anti-phosphothreonine (T)
- anti-nonmuscle myosin (M)
- anti-phosphoserine (S)
(all at 1:1000 dilution).
One blot was used for all three antibodies.
RBL-2H3 mast cells were sampled at 1, 2, 4 or 10min after activation.
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