Western blotting (4)

 

Micro-scale reagent delivery

Delivers picoliter volumes of reagent to a target surface using piezoelectric technology.

Application: Western blotting

Comparison between conventional Western blots and the new CHIP approach. CHIP has three clear advantages over conventional Western blotting
  • It uses far less primary antibody
  • It takes less time to achieve a result and the data is easy to interpret and visualize
  • It uses less membranes

 

Summary of results from western blotting experiment.
  • In unstimulated mast cells, there is little or no phosphorylation of the MHC on serine or threonine residues. After activation, the MHC becomes phosphorylated on both serine and threonine residues.
  • Both serine and threonine phosphorylation peak around 1-2 mins after activation, and then decreased, but the threonine phosphorylation remained for a longer period.
  • Using chemical printing technology to investigate protein phosphorylation with phosphospecific antibodies is much faster, requires fewer gels or blots and uses significantly less antibody when compared with conventional Western strategies.
  • Results obtained with the CHIP are very similar to those obtained using conventional Western blotting methods.
  • Where the sample can be limited or the source of the antibody is rare or expensive, the CHIP provides a powerful alternative to conventional Western blotting methods.
 

This page may contain references to products that are not available in your country.

Please contact us to check the availability of these products in your country.