Western blotting (4)
Micro-scale reagent delivery
Delivers picoliter volumes of reagent to a target surface using piezoelectric technology.
Application: Western blotting
Comparison between conventional Western blots and the new CHIP approach. CHIP has three clear advantages over conventional Western blotting
- It uses far less primary antibody
- It takes less time to achieve a result and the data is easy to interpret and visualize
- It uses less membranes
Summary of results from western blotting experiment.
- In unstimulated mast cells, there is little or no phosphorylation of the MHC on serine or threonine residues. After activation, the MHC becomes phosphorylated on both serine and threonine residues.
- Both serine and threonine phosphorylation peak around 1-2 mins after activation, and then decreased, but the threonine phosphorylation remained for a longer period.
- Using chemical printing technology to investigate protein phosphorylation with phosphospecific antibodies is much faster, requires fewer gels or blots and uses significantly less antibody when compared with conventional Western strategies.
- Results obtained with the CHIP are very similar to those obtained using conventional Western blotting methods.
- Where the sample can be limited or the source of the antibody is rare or expensive, the CHIP provides a powerful alternative to conventional Western blotting methods.
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